Understanding the regulation of genes in the organism is essential to understand the development and function of cells. The core of this process is the transcription factor (TFS). These proteins are helpful to open or close specific genes by binding to a specific area of DNA, which are called enhanced. Visualization in the nucleus has always been a major challenge. It requires complex technologies to see where these proteins are and how to interact. Now, a new technology can observe these protein interactions directly in the nucleus of fruit fly larvae glands, thereby emitting complex dances of molecules that drive gene regulation.
Researchers at Lyon University led by Dr. Samir MERABET and Dr. Solènevanderrere have developed a new experimental method with Dr. Solènevanderrerer and studied the protein interaction in the nucleus of Drosophila’s saliva glands. This new method is called BIFOR, combining biomolecular fluorescence complementarity (BIFC) with the bacterial anchor DNA label system. Their discovery has been published in the magazine.
Dr. MERABET explained that this new technology allows accurate quantity of diocyrin complexes on specific enhanced subcontane nucleus in fruit flies. The goal of the study is to explain the molecular tips of TF specificity in the body, which is the key to genetic regulation.
Researchers use BIFC, which is widely used to reveal protein-protein interaction (PPI) in various model systems including living fruit fly embryo. However, visual PPIs need to be visually visualized at the level of specific target enhanced sub -or genomic regions. The introduction of the anchor system can make these interaction accurate positioning and quantification without damaging the transcriptional adjustment.
The enhanced child with a good characteristic of the salivary glands selected device gene has obtained a significant result fork (Fkh250) As a model. The enhanced child is adjusted by the HOX protein comb and is related to the internal (EXD) auxiliary factor. Researchers have shown that the SCR/EXD complex is enriched on the FKH250 enhancer in the salivary gonad nucleus, which confirms the previous body and internal discovery.
The study shows that Parb1 -Macherry, a component of the BIFC signal and anchor system is significantly enriched, proves that the priority positioning on the anchor point Fkh250 Enhanced agent. The quantification of these signals indicates that the enrichment is specific to the SCR/EXD complex, because it has not observed a significant combination with another HOX/EXD complex or using SCR alone (required an EXD auxiliary factor to help SCR recognize its goals to identify its goals Fkh250 Enhancer).
By analyzing the other two variants, the sensitivity and specificity of Gemini technology further confirmed Fkh250 Enhanced agent: mutation version (Fkh250mut) And consensus version (Fkh250shortcomingTo. this Fkh250mut Augmented by a stronger, mutation eliminates the combination of HOX/EXD. There is no obvious BIFC signal enrichment, and Fkh250shortcoming The enhanced agent that allows different HOX/EXD complex recognition shows obvious enrichment.
This research sets the experimental foundation for the future application of the BIFOR strategy. This strategy can be applied to other organizations during the development of fruit flies and may be applied to other model creatures. These discoveries highlight the potential of BIFOR as the potential of the powerful tools of visualization and quantitative protein complex dynamics in specific DNA areas, thereby providing more in -depth insights for the molecular mechanism of genetic adjustment.
Dr. MERABET said: “Our work shows that Gemini can summarize the specific recognition of the target reinforcement of the target of the diocopin complex in the salivary gonad nucleus.” The method is designed to reveal the complexity of transcription factor interaction and genetic regulation.
The research by Dr. MEBET and Dr. Vanderperre marked the significant progress in the field of molecular biology and provided a new method to explore the dynamic interaction of protein in the nucleus. This innovation method has the potential of new aspects of revealing the specificity of genetic regulation and transcription factors, and paving the way for the development of biology and genetics in the future.
Journal reference
Vanderperre, Solène and Samir Merabet. “Diocoprotein complexes in the consolidation of the child in the salivary gonad nucleus of fruit flies larvae.” Cells, 2024. Doi: https: //doi.org/10.3390/cells13070613
About the author
Dr. Samir Merabet He is the director of CNRS (DE ReCherche Scientifique) of CNRS. He is a Ph.D. degree in Marseille’s doctorate degree in Marseille’s doctoral degree, at Biozentrum (IBDM, French IBDM) and postdoctoral works in Biozentrum. He established his own “individual occurrence and molecular interaction” in IGFL in 2012. Samir Merabet is always fascinated by conservative developmental regulators (HOX protein). His doctoral degree and post -doctoral work are committed to understanding his inherent molecular characteristics in development and evolution. Since his installation in IGFL, the Samir MERABET group is developing innovative tools to capture and study the protein interaction of the protein-protein interaction of other major development agents in other main model systems, including life, including life Fruit flies Embryo or larvae and human cells.
