Meiosis I of the Oocytes of C. elegans involves the dynamic interaction of cell structures, in which researchers discovered the key role of microtubules in membrane immersion during regulating polar in vivo extrusion. This process is crucial for maintaining genomic integrity during cell division, and the latest findings illuminate the balance of power required for successful polar body squeezes.
Professor Bruce Bowerman, as well as Alyssa Quiogun, Dr. Eisuke Sumiyoshi, Ph.D., Adam Fries, and Chien-Hui Chuang, from the Institute of Molecular Biology at the University of Oregon, have conducted a study showing how microtubulees can be associated with Actomyosin Cortex interacts to ensure how to interact with Actomyosin Cortex appropriate cell division in C. elegans oocytes. Their research, published in the peer-reviewed journal PLOS Genetics, provides new insights into the mechanisms of meiosis.
During C. elegans oocyte melanoma I, a contracting ring formed by cortical actin initiates membrane uptake, a critical step in polar body extrusion. Microtubules oppose this entry, creating a balance that prevents excessive deformation of the oocyte membrane, which is essential for proper chromosomal isolation. Professor Bowerman explained: “Our findings suggest that the microtubules themselves may provide a rigidity to counteract the forces produced by cortical actin, presumably doing some between each other or between cortical actin myosin Cross-linking, thus ensuring membrane uptake and not being regulated by regulatory cells.”
The researchers used advanced imaging techniques such as live-cell imaging and spin-disk confocal microscopy to label proteins with fluorescent labels (such as CLS-2, KNL-1 and BUB-1) to track their distribution and interactions in real time. This allows them to observe how the stability of the microtubule is affected by treatments such as nocozole and paclitaxel, which can affect membrane uptake. Professor Bowman noted: “Our experiments on paclitaxel and genetic backgrounds, which elevate cortical-associated microtubules, suggest that improved microtubules stability can inhibit membrane entrance defects seen in Cls-2 mutant oocytes , which suggests that promoting microtubule stability can promote inlet damage during mass degradation.
Major results of this study include observations that stabilizing or stabilizing microtubulees with nocozole or paclitaxel, respectively, would lead to invasive changes in the membrane. Specifically, instability leads to excessive invasion, while stability reduces it. These findings underscore the importance of well-regulated microtubule networks in cell division.
The study also highlighted that although actosin dynamics are crucial, fundamental microtubules may provide the structural support needed to balance membrane input, although indirect signaling from microtubules to actin has not been ruled out. This interaction between microtubule and actin, whether direct or indirect, is crucial to the correct function of the contraction ring of the kneaded pole body.
In summary, Professor Ballman and colleagues demonstrated the critical role of microtubules in relative corticoactin-driven membrane uptake during localized hypoplasia in C. elegans. This balance ensures oocytes Cortical stiffness to ensure sufficient stiffness to allow proper cell division and polar extrusion and polar extrusion, the A process is essential for developing the genetic stability of the embryo.
Journal Reference
Quiogue AR, Sumiyoshi E, Fries A, Chuang CH, Bowerman B. Microtubule against corticoactin-driven membrane-driven corticoactin-driven in C. elegans weight loss. PLOS gene. 2023. Doi:
